Primer dimers should be removed prior to sequencing if they comprise more than 5% of a library. We recommend pooling libraries and performing cleanup using a bead-based strategy following the ratio recommendations for the CUTANA™ DNA Purification Beads (EpiCypher 21-1407) (see the Technical Data Sheet on the product page). Cleanup should be performed on a normalized pool of sequencing libraries, NOT individual libraries, to minimize sample loss.
When should I remove primer dimers? What protocol do you recommend?
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