The CUTANATM CUT&RUN/Tag alignment pipeline requires sequencing libraries generated from paired-end sequencing to efficiently map reads back to the reference genome. Therefore, there should be two (2) FASTQ files for every reaction of CUT&RUN. On the platform, a Reaction is identified by its FASTQ Prefix, the portion of the FASTQ filename that is shared by only the forward and reverse read field (R1 & R2).
What is the difference between a FASTQ and a reaction?
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