Local Upload
FASTQ files, which contain raw sequencing data, can be seamlessly integrated into an Experiment within the platform. There are two primary methods for uploading these files: directly from your desktop during the initial Experiment setup phase, or at a later stage by accessing an already created Experiment.
For immediate upload during setup, users will find a designated "Add Data/FASTQs" section. Within this area, a highly intuitive "drag and drop" functionality is available, allowing for quick and effortless transfer of files from your desktop. Alternatively, users can opt for the "Browse" option, which will open a standard file directory interface, enabling them to navigate through their local storage and select the desired FASTQ files for upload.
This flexibility ensures that users can manage their data according to their workflow, whether they prefer to upload files immediately upon Experiment creation or integrate them as the project progresses. Both methods are designed for user-friendliness, streamlining the data input process.
BaseSpace Importer
For users that store FASTQ files on Illumina's BaseSpace server, a convenient direct import option is available. This feature facilitates the seamless transfer of your sequencing data directly from your linked BaseSpace account into CUTANATM Cloud, eliminating the need to download and upload large amounts of data.
To import, click the "BaseSpace Importer" button in the Add Data section of an Experiment , which will redirect you to the Illumina BaseSpace login page. After entering your credentials and logging in, return to the CUTANATM Cloud page. Here, you will see all your files sorted by Projects (entire sequencing runs) and Biosamples (individual reactions). In and BIOSAMPLES.Here, you will see a list of projects from your BaseSpace account. Select the checkbox for the desired files, then click "authorize" and "import." The files will be transferred in the background while you complete the Reaction sheet with the necessary information.
AWS Import
To import FASTQ files into a CUTANATM Cloud Experiment via Amazon Web Services, users need to provide their Role ARN and Bucket Name.
An Amazon Resource Name (ARN) is a unique global identifier for all AWS resources, acting as a comprehensive address across regions and accounts. A Role ARN identifies an AWS IAM role, which defines permissions for trusted entities to access resources using temporary credentials, essential for least privilege security.
In AWS, Buckets are top-level containers in Amazon S3, similar to folders. These scalable and secure object storage units logically group files and are tied to an AWS region, requiring a unique name within that region. Buckets are critical for managing access control, lifecycle policies, and configurations for their contained objects.
From Server
Upon choosing "From Server," you will be prompted to enter the specific URL associated with your secured server where the files are hosted. It is crucial to input the correct URL to ensure a successful connection.
Once the URL has been accurately entered, click on "Import Data." This action will initiate the download process, securely transferring your FASTQ files from your server to our platform. You can monitor the progress of the transfer, and a green notification will prominently appear on your screen once the files have been successfully uploaded and are ready for further analysis. This streamlined process ensures data integrity and convenience for users working with securely stored data.