Cleavage Under Targets & Tagmentation (CUT&Tag) is a groundbreaking epigenomic mapping strategy that builds on its predecessor immunotethering technologies CUT&RUN and ChIC. In CUTANA™ CUT&Tag, nuclei are immobilized to a solid support and antibodies bind their chromatin targets in situ. A fusion of proteins A and G with prokaryotic transposase 5 (pAG-Tn5) is used to selectively cleave and tagment antibody-bound chromatin with sequencing adapters (see Figure 1). Tagmented fragments are directly PCR amplified using EpiCypher’s exclusive single-tube (“Direct-to-PCR”) approach, yielding sequence-ready DNA.
The CUTANA CUT&Tag Kit is ideal for genomic mapping experts that want to increase throughput and scale without sacrificing sensitivity. Our unique single-tube workflow is designed for multi-channel pipetting and can be completed in just two days. These features, combined with reduced assay costs and exquisite signal-to-noise, position CUT&Tag to become a leading tool for chromatin research.
Note: CUT&Tag is best for mapping histone post-translational modifications (PTMs). For chromatin-associated proteins, CUT&RUN assays are strongly recommended.
Figure 1: CUTANA CUT&Tag workflow schematic.