The CUTANA ATAC-seq Kit provides multiple quality control checks to ensure consistent generation of robust open chromatin profiles (Figure 1 and Figure 2), including established ATAC-seq success metrics, to support reliable ATAC-seq workflows [1]. To assess ATAC-seq library quality based on enrichment of accessibility signals around Transcription Start Sites (TSSs), calculate the TSS enrichment score according to ENCODE recommendations as a functional signal-to-background measure.
Figure 1. Success metrics and optimization guidelines.
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Figure 2. Representative open chromatin profiles and QC metrics generated by a robust, well-established ATAC-seq workflow. Results demonstrate highly reproducible peak profiles, strong FRiP scores, and high TSS enrichment across experiments