Adapter dimers should be removed when they comprise >5% of your library (i.e. % Integrated in trace is less than 5%), as determined by TapeStation or Bioanalyzer. We recommend using the optimized ratio for adapter dimer removal in the Application Notes for our CUTANA™ DNA Purification Beads (EpiCypher 21-1407). Note that this ratio removes adapter dimers from pooled sequencing libraries, as opposed to individual sequencing libraries, which helps reduce sample loss.
When should I remove adapter dimers? What protocol do you recommend?
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