EpiCypher offers select histone PTM antibodies validated for robust CUT&Tag profiling. Visit our website for the most up-to-date list.
Histone PTM antibodies are particularly susceptible to off-target binding, which can compromise biological interpretations. To address these problems, EpiCypher developed the SNAP-CUTANA™ K-MetStat Panel (EpiCypher 19-1002), and is using these defined nucleosome spike-in controls to identify best-in-class histone lysine methylation PTM antibodies for CUT&Tag. This strategy is the only method that directly confirms antibody specificity in CUT&Tag against physiological on- and off-target substrates. We also validate antibody efficiency, allowing users to be confident when using reduced cell numbers. Each of our SNAP-Certified™ Antibodies show:
High target specificity: <20% recovery of off-target PTMs in the K-MetStat Panel.
High efficiency: Robust profiling at 100,000 and 10,000 K562 nuclei.
If your target has not been validated in CUT&Tag, you can try an antibody that has been validated in CUT&RUN. Although we cannot guarantee that CUT&RUN antibodies will work in CUT&Tag, they are a good starting point as they have also been rigorously tested by EpiCypher. If we do not carry a CUT&Tag or CUT&RUN validated antibody for your target, you will need to validate antibodies from external vendors; contact us for recommendations.