Adapter dimers should be removed when they comprise >5% of your library (i.e. % Integrated in trace is less than 5%), as determined by TapeStation or Bioanalyzer. We recommend using the optimized ratio for adapter dimer removal in the Application …

This article also applies to meCUT&RUN and Multiomic CUT&RUN. EpiCypher’s CUTANA TM Wash Buffer Enhancers ( EpiCypher 14-1803 ) are optimized additives for all CUTANA TM assays that reduce sample clumping, a frequent cause of poor sequencing performance …

This article also applies to meCUT&RUN and Multiomic CUT&RUN. Important Notes High-quality sample prep is essential for CUT&RUN experimental success. This guide is for fresh, native (i.e. unfixed, not frozen) suspension cell culture. For alternative …

Note that EpiCypher does NOT recommend using library yields to determine assay success, as yields can vary by cell type, number of cells, target abundance, antibody quality, and assay type (please note that this kit is compatible with more than …

Important Notes Trypan Blue is toxic to cells. After adding Trypan Blue dye to cells, move quickly to determine cell viability. Materials needed Reagent Trypan Blue solution, 0.4% (e .g. Invitrogen T10282) Hemocytometer with brightfield/phase microscope or automated …

For DIY CUT&Tag customers (i.e., those using individual EpiCypher CUT&Tag reagents, NOT using our all-in-one CUT&Tag Kits), you can download the latest DIY CUT&Tag protocol here .

This article also applies to Multiomic CUT&RUN. EpiCypher offers a collection of antibodies proven to generate robust CUT&RUN profiling data. Visit our antibodies complete list for the most up-to-date list. We continue to investigate additional antibodies …

This article also applies to meCUT&RUN and Multiomic CUT&RUN. We do not recommend using TapeStation/Bioanalyzer or qPCR to evaluate raw CUT&RUN DNA, as they will NOT provide useful information at this step of the workflow. CUT …

This article also applies to meCUT&RUN and Multiomic CUT&RUN. Important Notes High quality sample prep is essential to CUT&RUN assay success and is the main variable we see when troubleshooting customer experiments. To ensure high quality sample …

This article also applies to meCUT&RUN and Multiomic CUT&RUN. To determine the quality of freeze/thawed cells for CUTANA ™ assays, consider the following criteria: Cell morphology and integrity. Do cells appear rounded with intact membranes? What is …

Adapter dimers are generated by self-ligation of sequencing adapters that are preferentially amplified due to their small size. Adapter dimer contamination appears as a peak at ~150 bp (below), and is caused by low input DNA, inefficient adapter ligation …

This article also applies to Multiomic CUT&RUN. We recommend selecting the antibody that best balances the need for robust DNA yields, enrichment for expected sequence motifs and/or peak structures, and high signal over background. Testing native and lightly …