SNAP-CUTANA™ Spike-ins are the ONLY control that can monitor CUTANA™ assay success, flag failed reactions, and identify potential causes for troubleshooting. SNAP-CUTANA Spike-in Controls can also be used to validate antibody specificity in the context of your CUT&RUN and CUT&Tag workflow. Together, these controls allow researchers to be confident in their experimental results.
Should you reach out to our technical support team for troubleshooting assistance, they will be able to assist you much better if you include data derived from this panel.
When should I use the SNAP-CUTANA Spike-ins?
EpiCypher pairs the SNAP-CUTANA K-MetStat Panel with our validated H3K4me3 and H3K27me3 positive control antibodies and IgG negative control antibodies in every CUT&RUN/CUT&Tag experiment, and it has saved us countless time and resources in protocol optimization. For details on how to leverage the K-MetStat Panel for troubleshooting, see this article.
SNAP-CUTANA Spike-ins can also be used to validate histone PTM antibodies, as detailed in this article.
How does it compare to other spike-in controls?
Although the SNAP-CUTANA K-MetStat Panel works like other genomic spike-ins, its design comes with added benefits. The K-MetStat Panel is the only control that uses purified recombinant nucleosomes, replicating the physiological target of chromatin mapping assays. The panel comprises 16 unique nucleosomes, each containing a distinct histone lysine methylation PTM (or unmodified) and DNA barcode, which provides both on- and off-target substrates for control reactions.
Are SNAP-CUTANA Spike-ins easy to use?
Yes! The K-MetStat Panel is directly added to cells in one step, and processed alongside the sample throughout CUT&RUN and CUT&Tag protocols (see Figure 1 below). No other assay modifications are necessary.
The spike-in data can be used to validate specific aspects of the workflow, such as antibody specificity or enzyme activity, and provide key insights for troubleshooting. For information on how to use the K-MetStat Panel for workflow validation and monitoring technical variability, see this article.
Figure 1. SNAP-CUTANA Spike-in Controls are processed alongside samples for a direct, in-assay readout of experimental success.