CUTANA™ Fiber-seq is a long-read, multiomic sequencing assay that simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants. Unlike short-read methods, Fiber-seq provides base-resolution chromatin maps in repetitive and structurally complex regions of the genome. For more background, see the groundbreaking 2020 Nature article by Andrew Stergachis introducing Fiber-seq.
Key Benefits:
Multiomic profiling in a single assay
Antibody-free, PCR-free workflow
Compatible with PacBio and Oxford Nanopore (ONT) sequencing
Resolves nucleosomes, transcription factor binding, and RNA polymerase footprints at near-nucleotide precision
Simple upstream workflow (<3 hours before library prep)
Powered by Hia5, the highest-activity N6-adenine methyltransferase (6mA MTase), Fiber-seq enables fast and efficient labeling of accessible DNA prior to long-read sequencing. Purchase Hia5 (EpiCypher 15-1032-8rxn / EpiCypher 15-1032-24rxn) exclusively from EpiCypher for DIY Fiber-Seq, or use our Fiber-Seq Kit (EpiCypher 14-2001-8rxn or 14-2001-24rxn) for an integrated solution that takes you from nuclei isolation through gDNA purification.
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Figure 1: Fiber-seq simultaneously detects open chromatin (marked by 6mA) and DNA methylation (5mC) in human leukemia (K562) cells. ATAC-seq and CTCF CUT&RUN data are shown for comparison. Each horizontal line in the Fiber-seq panels represents a single long read, providing single-molecule data. Data from 60 individual DNA molecules are shown.