EpiCypher offers a collection of antibodies proven to generate robust CUT&RUN profiling data. Visit our antibodies complete list for the most up-to-date list.
We continue to investigate additional antibodies for high-quality performance in CUT&RUN. Below we outline the lot-specific testing criteria for EpiCypher CUTANA™ CUT&RUN antibodies for various target classes.
Chromatin-associated protein targets: We offer CUT&RUN antibodies to transcription factors (e.g. CTCF), chromatin reader proteins (e.g. BRD4), modifying enzymes (e.g. MLL1), and remodelers (e.g. SMARCA2, SMARCA4). Each antibody displays high signal-to-noise in CUT&RUN and generates genomic distribution profiles consistent with the reported function of the target protein (for example, DNA binding motif analysis for transcription factors).
Epitope-tagged protein targets: Our antibodies to HA- and DYKDDDDK-tagged proteins are validated in CUT&RUN using defined SNAP-CUTANA™ Spike-in Controls. See this blog for more information about these antibodies, their spike-in controls, and how they can be used for robust CUT&RUN profiling.
Histone PTM targets: Histone PTM antibodies are particularly susceptible to off-target binding, which can compromise biological interpretations. To address these problems, EpiCypher developed the SNAP-CUTANA K-MetStat Panel (EpiCypher 19-1002), and is using these defined nucleosome spike-in controls to identify best-in-class histone lysine methylation PTM antibodies for CUT&RUN. This strategy is the only method that directly confirms antibody specificity in CUT&RUN against physiological on- and off-target substrates. We also validate antibody efficiency, allowing users to be confident when using reduced cell numbers. Each of our SNAP-Certified™ Antibodies show:
High specificity: <20% recovery of off-target PTMs in the K-MetStat Panel.
High target efficiency: Robust profiling at 500,000 and 50,000 starting cells.