Does CUT&RUN require adjustments for transcription factors or other chromatin proteins?

No, CUT&RUN for transcription factors and other chromatin proteins can be completed using the most recent standard manual. In particular, you can use the recommended 1.8X CUTANA^TM DNA Purification Beads ratio (EpiCypher 21-1407) for all targets.

If you routinely profile transcription factors in CUT&RUN, you may consider using our validated CTCF antibody (EpiCypher 13-2014) as a positive control, in addition to the H3K4me3 and H3K27me3 positive controls provided with the CUTANA CUT&RUN Kit.

If you are unsure what results to expect for your target, reach out to EpiCypher Tech Support for guidance.