Sample prep for adherent cells

Collect adherent cells using a mild Trypsin digestion, which dislodges and disaggregates clumps into monodispersed cells without cell damage.

  1. Incubate with 0.05% Trypsin at 37°C for the minimal time necessary to dislodge cells.
  2. Add pre-warmed complete media to inactivate Trypsin and then collect cells. Trypsin will be removed during subsequent wash steps.
  3. Proceed to initial cell count and harvest cells for CUT&RUN.

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