Sample prep for adherent cells
Collect adherent cells using a mild Trypsin digestion, which dislodges and disaggregates clumps into monodispersed cells without cell damage.
- Incubate with 0.05% Trypsin at 37°C for the minimal time necessary to dislodge cells.
- Add pre-warmed complete media to inactivate Trypsin and then collect cells. Trypsin will be removed during subsequent wash steps.
- Proceed to initial cell count and harvest cells for CUT&RUN.