---
title: "When should I remove primer dimers? What protocol do you recommend?"
slug: "primer-dimer-removal-questions"
updated: 2025-07-14T21:16:07Z
published: 2025-07-15T12:00:29Z
canonical: "support.epicypher.com/primer-dimer-removal-questions"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# When should I remove primer dimers? What protocol do you recommend?

Primer dimers should be removed prior to sequencing if they comprise more than 5% of a library. We recommend pooling libraries and performing cleanup using a bead-based strategy following the ratio recommendations for the CUTANA™ DNA Purification Beads ([EpiCypher 21-1407](http://www.epicypher.com/product/cutana-dna-purification-beads/)) (see the Technical Data Sheet on the product page). Cleanup should be performed on a normalized pool of sequencing libraries, NOT individual libraries, to minimize sample loss.