---
title: "When should I remove adapter dimers? What protocol do you recommend?"
slug: "when-should-i-remove-adapter-dimers"
updated: 2025-07-15T14:40:48Z
published: 2025-07-15T14:40:48Z
canonical: "support.epicypher.com/when-should-i-remove-adapter-dimers"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# When should I remove adapter dimers? What protocol do you recommend?

Adapter dimers should be removed when they comprise >5% of your library (i.e. % Integrated in trace is less than 5%), as determined by TapeStation or Bioanalyzer. We recommend using the optimized ratio for adapter dimer removal in the Application Notes for our CUTANA™ DNA Purification Beads ([EpiCypher 21-1407](http://www.epicypher.com/product/cutana-dna-purification-beads/)). Note that this ratio removes adapter dimers from **pooled** sequencing libraries, as opposed to individual sequencing libraries, which helps reduce sample loss.