Looking to set up CUT&RUN in your lab? This page outlines the key products you’ll need to purchase for a successful CUT&RUN experiment. Whether you're starting from scratch or restocking, you'll find quick links to purchase each component and get your experiment up and running.
The specific items you’ll need depend on whether you’re using our all-in-one CUT&RUN Kit or following our step-by-step DIY Protocol. Kit users will receive most of the core reagents in their kit box, but a few additional items must be purchased separately. If you’re following the DIY route you’ll need to assemble all components individually, including the pAG-MNase enzyme, buffers, and other essential reagents. We’ve outlined both options below to help you quickly find what fits your workflow.
Kit Users
Required EpiCypher reagents:
Item | EpiCypher SKU | Why do I need this? |
CUTANA™ CUT&RUN Library Prep Kit | ||
Magnetic Separation Rack, 0.2 mL Tubes | ||
CUTANA™ Protease Inhibitor Tablets |
Additional EpiCypher reagents you may need:
Item | EpiCypher SKU | Needed when: |
CUTANA™ CUT&RUN Library Prep Kit | A library prep kit of some form is required—if using another kit, please reach out to techsupport@epicypher.com for key protocol modifications | |
SNAP-CUTANA™ K-MetStat Panel | You intend to do more than 10 positive or negative control reactions | |
CUT&RUN Antibodies | Your experimental target is one we provide an antibody to | |
Magnetic Separation Rack, 1.5 mL Tubes | Preparing cells/reactions in bulk | |
CUTANA™ Nuclei Extraction Buffer | ||
Additional SPRI beads or DNA spin column kit (e.g. Monarch® Spin PCR & DNA Cleanup Kit, NEB T1130) | Targeting chromatin proteins that generate small fragments (<120 bp) |
DIYers
Required EpiCypher reagents:
Item | EpiCypher SKU | Why do I need this? |
Concanavalin A (ConA) Conjugated Paramagnetic Beads | 21-1401 | |
CUTANA™ pAG-MNase | 15-1016 or 15-1116 | |
SNAP-CUTANA™ K-MetStat Panel | 19-1002 | |
Rabbit IgG Negative Control Antibody | 13-0042 | |
H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag | 13-0060 | |
H3K27me3 Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag | 13-0055 | |
E. coli Spike-in DNA | 18-1401 | |
CUTANA™ Quick Cleanup DNA Purification Kit | 14-0052 | |
CUTANA™ CUT&RUN Library Prep Kit | ||
Magnetic Separation Rack, 0.2 mL Tubes | ||
CUTANA™ Protease Inhibitor Tablets |
Additional EpiCypher reagents you may need:
Item | EpiCypher SKU | Needed when: |
CUTANA™ CUT&RUN Library Prep Kit | A library prep kit of some form is required—if using another kit, please reach out to techsupport@epicypher.com for key protocol modifications | |
SNAP-CUTANA™ K-MetStat Panel | You intend to do more than 10 positive or negative control reactions | |
CUT&RUN Antibodies | Your experimental target is one we provide an antibody to | |
Magnetic Separation Rack, 1.5 mL Tubes | Preparing cells/reactions in bulk | |
CUTANA™ Nuclei Extraction Buffer | ||
Additional SPRI beads or DNA spin column kit (e.g. Monarch® Spin PCR & DNA Cleanup Kit, NEB T1130) | Targeting chromatin proteins that generate small fragments (<120 bp) |