---
title: "What control antibodies and spike-ins should I use in CUT&Tag?"
slug: "what-controls-should-i-use-cut-and-tag"
tags: ["cut and tag"]
updated: 2024-12-03T21:52:18Z
published: 2024-12-03T21:52:18Z
canonical: "support.epicypher.com/what-controls-should-i-use-cut-and-tag"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# What control antibodies and spike-ins should I use in CUT&Tag?

EpiCypher has specific recommendations for control antibodies and spike-ins controls in CUT&Tag experiments, outlined below. Note that these controls are included in the CUTANA™ CUT&Tag Kit; for DIY CUT&Tag protocol users, links to relevant products are provided.

## **Control antibodies paired with SNAP-CUTANA™ K-MetStat Panel**

Reactions using negative control (IgG) and positive control (H3K4me3, H3K27me3) antibodies should be included in every experiment to validate protocols and examine assay background. When paired with the SNAP-CUTANA Spike-in K-MetStat Panel of nucleosome spike-in controls, control reactions can assist in [validating](https://support.epicypher.com/docs/snap-cutana-controls-assay-success-cut-and-tag) and [troubleshooting](https://support.epicypher.com/docs/using-snap-cutana-spike-ins-to-troubleshoot-workflows-cut-and-tag) your workflow.

- H3K4me3, [EpiCypher 13-0060](https://www.epicypher.com/products/antibodies/h3k4me3-antibody-snap-certified-for-cut-run-and-cut-tag)
- H3K27me3, [EpiCypher 13-0055](https://www.epicypher.com/products/antibodies/h3k27me3-antibody-snap-certified-for-cut-run-and-cut-and-tag)
- IgG, [EpiCypher 13-0042](https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls/cutana-igg-negative-control-antibody-for-cut-run-and-cut-tag)
- SNAP-CUTANA K-MetStat Panel, [EpiCypher 19-1002](https://www.epicypher.com/products/nucleosomes/snap-cutana-k-metstat-panel)

## **Spike-in nucleosome controls**

SNAP-CUTANA™ Spike-in Controls are the only control that uses purified recombinant nucleosomes, replicating the physiological target of CUT&Tag assays.These panels of on- and off-target spike-ins can be used to validate antibodies to experimental targets, including lysine methylation PTMs and epitope-tagged proteins. For more information on SNAP-CUTANA Spike-in Controls, navigate to specific articles on:

- [What are SNAP-CUTANA Spike-in Controls and how do they work?](https://support.epicypher.com/docs/what-are-snap-cutana-spike-ins-cut-and-tag)
- [Analyzing SNAP-CUTANA Spike-in Controls](https://support.epicypher.com/docs/analyzing-snap-cutana-spike-in-controls-and-results-cut-and-tag)
- Using SNAP-CUTANA Spike-in Controls to [determine CUT&Tag success](https://support.epicypher.com/docs/snap-cutana-controls-assay-success-cut-and-tag)
- Using SNAP-CUTANA Spike-in Controls to [troubleshoot workflows](https://support.epicypher.com/docs/using-snap-cutana-spike-ins-to-troubleshoot-workflows-cut-and-tag) or [validate select histone PTM antibodies](https://support.epicypher.com/docs/snap-cutana-histone-ptm-antibody-validation-cut-and-tag)