---
title: "How many sequencing reads are needed for CUT&Tag?"
slug: "sequencing-reads-cut-and-tag"
updated: 2024-12-04T14:15:07Z
published: 2024-12-04T14:15:07Z
canonical: "support.epicypher.com/sequencing-reads-cut-and-tag"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# How many sequencing reads are needed for CUT&Tag?

Libraries should be sequenced to a depth of 5-8 million total reads. For sufficient coverage, each library should generate 3-5 million **unique** reads (after removing multi-mapping reads, duplicate reads, reads in DAC exclusion list regions).