---
title: "How Many Cells Are Needed for CUT&RUN? | Input Recommendations"
slug: "number-cells-cut-and-run"
description: "Learn how many cells are recommended for CUT&RUN, minimum input requirements, and tips for optimizing low-input experiments."
updated: 2026-02-27T14:56:16Z
published: 2026-02-27T14:56:16Z
canonical: "support.epicypher.com/number-cells-cut-and-run"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# How many cells do I need for CUT&RUN?

> This article also applies to meCUT&RUN and Multiomic CUT&RUN.

For CUTANA™ CUT&RUN assays, we recommend harvesting 500,000 cells per reaction plus 10% excess to account for sample loss.

If your experiment requires fewer cells, see [this article](/v1/docs/optimize-cut-and-run-targets-cells) for more information.