---
title: "Low Positive Control Yields in CUT&RUN | Did My Experiment Fail?"
slug: "my-yields-from-the-positive-control-are-low-did-my-experiment-fail"
description: "Learn what low positive control yields mean in CUT&RUN, when to troubleshoot, and how to determine whether your experiment succeeded."
updated: 2024-10-02T15:47:25Z
published: 2024-10-02T15:47:25Z
canonical: "support.epicypher.com/my-yields-from-the-positive-control-are-low-did-my-experiment-fail"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# My yields from the H3K4me3 positive control are low (i.e. below 5 ng), about the same as the IgG negative control. Did my experiment fail?

Similar yields from H3K4me3 and IgG controls do NOT imply CUT&RUN failure. H3K4me3 is a low abundance target, resulting in lower yields that are often similar to IgG. In these cases, use the total amount of CUT&RUN-enriched DNA for library prep. In EpiCypher's experience, good sequencing data with high signal-to-noise are still obtained.

If desired, a high abundance target (e.g. H3K27me3) can be used as an additional positive control, as yields will be much higher than IgG.