---
title: "How stable is CUTANA™ Tn5 Transposome? Will multiple freeze/thaws of from storage at -80ºC lead to lost activity? Should I aliquot the enzyme?"
slug: "how-stable-is-cutana-tn5-transposome"
status: "new"
updated: 2026-09-15T01:28:27Z
published: 2026-09-15T01:28:27Z
canonical: "support.epicypher.com/how-stable-is-cutana-tn5-transposome"
---

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# How stable is CUTANA™ Tn5 Transposome? Will multiple freeze/thaws of from storage at -80ºC lead to lost activity? Should I aliquot the enzyme?

The CUTANATM Tn5 Transposome has no observable loss of activity for up to 10 cycles of freeze-thaw when frozen at -80ºC and thawed on ice. If you anticipate accessing this aliquot more than 10 times, make smaller aliquots the first time it is thawed. Long-term storage at temperatures ≥4ºC is not recommended and will result in observable loss of activity within days to weeks.