---
title: "Can I use E. coli DNA for CUT&Tag sequencing normalization?"
slug: "e-coli-dna-cut-and-tag"
updated: 2024-12-04T14:07:11Z
published: 2024-12-04T14:07:11Z
canonical: "support.epicypher.com/e-coli-dna-cut-and-tag"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Can I use E. coli DNA for CUT&Tag sequencing normalization?

CUTANATM pAG-Tn5 is highly purified and depleted of contaminating nucleic acids, so residual *E. coli* DNA cannot be used for sequencing normalization. We do not recommend adding exogenous *E. coli* Spike-in DNA, as we have not thoroughly optimized its use in CUT&Tag.