---
title: "Does Hia5 labeling affect sequencing quality?"
slug: "does-hia5-affect-sequencing-quality"
updated: 2026-05-13T17:19:24Z
published: 2026-05-13T17:19:24Z
canonical: "support.epicypher.com/does-hia5-affect-sequencing-quality"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Does Hia5 labeling affect sequencing quality?

No, Hia5 labeling does not affect sequencing quality. 6mA labeling is a **non-destructive reaction** and as such read lengths remain unchanged compared to untreated DNA. You may notice slight differences in read quality, but these are due to basecaller performance rather than any damage to the DNA.