---
title: "Do I Need to Sequence an Input Control for CUT&RUN?"
slug: "do-i-need-to-sequence-an-input-control"
description: "Learn why CUT&RUN does not require an input control and which controls to sequence instead for accurate quality control and analysis."
updated: 2024-09-05T18:18:49Z
published: 2024-09-05T18:18:49Z
canonical: "support.epicypher.com/do-i-need-to-sequence-an-input-control"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Do I need to sequence an input control?

**NO, you do not need to sequence an input control.** Because there is no immunoprecipitation step in CUTANA CUT&RUN, CUT&Tag, and related immunotethering assays, there is no input to sequence.

However, we do recommend including a reaction with the [IgG negative control antibody](https://www.epicypher.com/products/nucleosomes/snap-cutana-spike-in-controls/cutana-rabbit-igg-cut-run-negative-control-antibody), and sequencing this reaction in every experiment. This control will help gauge assay background.