---
title: "DIY CUT&RUN Protocol Without a Commercial Kit"
slug: "cutrun-diy-protocol"
description: "Perform CUT&RUN without a commercial kit using this DIY protocol, Epicypher's reagent list, workflow, controls, and optimization recommendations."
updated: 2026-08-11T13:26:09Z
published: 2026-08-11T13:26:09Z
canonical: "support.epicypher.com/cutrun-diy-protocol"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# CUT&RUN DIY Protocol (Non-Kit Users)

For DIY CUT&RUN customers (i.e., those using individual EpiCypher CUT&RUN reagents, NOT using our all-in-one CUT&RUN Kits), you can download the latest DIY CUT&RUN protocol [here](https://cdn.document360.io/1fc69f91-cf1e-45ab-bc51-4063eb80e37c/Images/Documentation/2026.08.03_CUTANA%20CUT&RUN%20Protocol_v3.1.pdf).