---
title: "Considerations when sequencing low-concentration CUT&Tag libraries"
slug: "cut-and-tag-sequence-low-concentration"
tags: ["cut and run"]
updated: 2024-12-09T17:26:57Z
published: 2024-12-09T17:26:57Z
canonical: "support.epicypher.com/cut-and-tag-sequence-low-concentration"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://support.epicypher.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Considerations when sequencing low-concentration CUT&Tag libraries

Ideally, you would [troubleshoot low CUT&Tag yields](https://support.epicypher.com/docs/troubleshooting-cut-and-tag-yields#troubleshooting-summary), repeat the experiment, and then proceed to sequencing. This will result in higher quality sequencing data compared to using ultra-low CUT&Tag yields. However, in some cases, repeating the experiment isn’t possible. In fact, for some targets and cell types, low CUT&Tag yields are unavoidable.

**If it is not possible to repeat the experiment**, use a Speedvac to increase library concentration and add as much of the library as possible to the sequencing pool. Deeper sequencing (> 8 million total reads) is recommended to ensure sufficient read depth and fully capture library diversity.

Read duplicates [may be increased](/v1/docs/high-read-duplicates-cut-and-tag) but can be filtered out with Picard ([broadinstitute.github.io/picard](https://broadinstitute.github.io/picard/)).