Can I use my ChIP antibody for CUTANA™ assays?

You may try using ChIP antibodies for CUTANA assays, but fair warning: EpiCypher has found that success in ChIP does NOT guarantee success in CUT&RUN, CUT&Tag, or related chromatin mapping assays.

This is largely due to differences in sample prep and processing steps (see this blog for information). ChIP uses heavily cross-linked cells, stringent wash buffers, and bead-coupled antibodies to help maximize the signal-to-noise ratio. However, these strategies often lead to loss of on-target signal, which is particularly problematic for low abundance targets. To counteract these effect, ChIP requires highly efficient antibodies, with high yields.

In contrast, CUT&RUN uses native chromatin, mild washes, and antibodies in solution, reflecting the increased sensitivity of this newer technique. The only way to know if your antibody will work in CUTANA assays if the antibody has been tested in this assay - antibody validation using ChIP, immunoblot, ELISA, IHC, or other techniques is NOT a predictor of CUTANA performance.

For your convenience, EpiCypher offers CUT&RUN- and CUT&Tag-validated antibodies, which you can shop here.