What should I expect when using frozen cell samples for CUT&RUN?
It is normal to lose viability following freeze-thaw, as cells may be more permeable to Trypan Blue. For frozen samples, EpiCypher scientists often focus more on cellular morphology. Total cell counts are important, as is the balance between good cell integrity vs. cell lysis. As long as you have not lost a substantial portion of cells, and there is minimal cell lysis, you should be fine to move forward with CUT&RUN.