Sample prep for tissues

Tissues must be processed into a monodispersion of cells, typically by mechanical maceration or douncing. Enzymatic digestion (e.g. collagenase, dispase) can be used for connective tissue and Trypsin may be used for macro-dissected tissues (monitor dissolution to single cells). Harvesting viable, monodispersed cells from tissues can be challenging; in some cases nuclei may be preferable. See literature, including the following papers, for additional methods:

Did this answer your question? Thanks for the feedback There was a problem submitting your feedback. Please try again later.